WIV04 is representative of and identical to the early outbreak sequences. WIV04 was isolated by the Wuhan Institute of Virology from a clinical sample of a bronchoalveolar lavage fluid (BALF) collected at the Wuhan Jinyintan Hospital in Hubei Province on 30th December 2019 from a symptomatic patient, a retailer working at the Huanan Seafood Wholesale Market.
BALF was used for RNA extraction and metagenomic next-generation sequencing (NGS). The consensus sequence was obtained by de-novo assembly. (Zheng-Li Shi et al 2020)